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Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Corning Life Sciences transwell tissue culture plate with pore polyester membrane insert #3470
Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Corning Life Sciences cell culture transwells 3.0-mm pore-size insert, polyester membrane, dimension
Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Becton Dickinson transwell polyester membrane cell culture inserts
Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Corning Life Sciences microporous filters transwell® polyester membrane cell culture inserts
Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Corning Life Sciences polyester (pet) membrane transwell clear inserts diameter pore size; tissue culture treated corning 3450
Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Corning Life Sciences transwell co-culture system millicell small suspension cell culture insert, polyester membrane, 6 wells
Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Corning Life Sciences transwell tissue culture inserts with polyester membranes of 5- m pore diameter
Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to <t>Transwell</t> exposure to D . tertiolecta .
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Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to Transwell exposure to D . tertiolecta .

Journal: PLoS ONE

Article Title: Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays

doi: 10.1371/journal.pone.0201438

Figure Lengend Snippet: Relative Hsp70b mRNA expression in CHSE-214 cells exposed to H . akashiwo (CCMP302) (10,000 cell/mL) for 6 h in the exponential (A) and stationary (B) growth phases. Data were relativized to Transwell exposure to D . tertiolecta .

Article Snippet: The ichthyotoxic microalga ( H . akashiwo ) was co-cultured with the CHSE-214 cell line by using a Transwell culture system with 0.4 μm pore polyester membrane inserts (Corning HTS Transwell).

Techniques: Expressing